SMILEK Jiří Brno University of Technology

New Approach for Characterization and Study on Reactivity of Biomaterials

Co-authors SEDLÁČEK Petr, KLUČÁKOVÁ Martina

New method for study on reactivity and transport ability of biomaterials based on combination of innovative diffusion techniques and advantages of hydrogels was developed. Stationary penetration experiments (diffusion cells) were applied for biomaterials (anionic humic acids and cationic chitosan) in supported hydrogel matrix. Linear polysaccharide – agarose – was used as non-reactive matrix, which was characterized from mechanical and structural point of view. The reactivity and transport properties of chosen biomaterials were studied by the interactions with simple organic dyes (Methylene Blue C.I. 52015 and Chicago Sky Blue 6B C.I. 24410). The rate of interaction of organic dyes with biomaterials in the case of stationary experiments (break-through technique) was compared by fundamental diffusion parameters (effective diffusion coefficient, lag time and binding capacity). Results obtained from stationary diffusion technique clearly illustrate the immobilizing effect of chitosan (humic acids, respectively) on the transport of organic dye in hydrogel. The influence of concentration of biomaterials on the transport of organic dye and the reactivity of biomaterials was studied as well. Presented diffusion technique (together non-stationary diffusion techniques) in combination with excellent transport properties of hydrogels (diffusion is not disturbed by convection, characterization can be done very easily, etc.) seem to be suitable universal preliminary reactivity-mapping tool for wide range of biomaterials at changing experimental conditions.