PILAŘOVÁ Kateřina Technical University of Liberec

Drawing Fibrous Scaffolds with Precise Structure for Tissue Engineering

Co-authors JENČOVÁ Věra, STANISLAV Lukáš, HORÁKOVÁ Jana, MIKEŠ Petr, LUKÁŠ David

This work is focused on evaluation of scaffolds made by drawing technique for the cell adhesion, viability and proliferation. With drawing we are able to produce very precise fibres with specified orientation from different polymers (PCL, PVA, PVB and others). This characteristic could be an advantage in tissues with ordered pattern. In the primary study the handmade fibres were tested for the cell growth and the results showed the potential of the cells to overgrow the gaps between the fibres. On account of these results further tests were necessary to be done with the precise machine-made fibrous scaffolds. The machine-made fibres are thinner, the gaps between the fibres are smaller and the whole structure is denser. In this study we focused on a planar scaffolds with fibres ordered either in one, in two or in three directions. Mice fibroblasts (3T3 Swiss Albino) were used for the in vitro experiment. The scaffolds were tested at day 1, 3, 7, 14, 21 and 28 after cell seeding. MTT assay, fluorescent microscopy and SEM analysis were used for the evaluation of cell adhesion, viability and proliferation. The results from microscopy analysis as well as from the MTT assay show, that the machine-made scaffolds are capable of supporting cellular attachment and proliferation during our in vitro tests. The scaffolds with crossed fibres show better results compared to fibres ordered in one direction. Nevertheless the one-direction fibres have a great potential in many tissue engineering applications, e.g. for muscles or neural tissue.

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