UKRAINTSEV Egor Institute of Physics of the ASCR, v.v.i.

Computational Study of Cellular Assembly on Hydrophobic/Hydrophilic Micro-patterns

Co-authors BROZ Antonin, KALBACOVA Marie, KROMKA Alexander, REZEK Bohuslav

Cell migration and assembly play an important role in many biological systems. We develop simple one-dimensional stochastic model of cell behavior on chemically patterned surfaces that is based on three key parameters: speed of cell movement (motility) across substrate, probability of cell adhesion to substrate, and probability of cell division on substrate when adhered on substrate. Amount of adhered cells on hydrophobic and hydrophilic regions is calculated as function of time (number of cycles up to 2000). The model is correlated with in-vitro data obtained within 48 h in real time. We show that this simple stochastic model with the three parameters (where cell motility is the most important one) can describe with high accuracy the experimental data and thereby explain the observed preferential cell assembly on hydrophilic/hydrophobic micro-patterns (up to 200 um width).