BRZICOVÁ Táňa Institute of Experimental Medicine AS CR v.v.i.

Cytotoxicity Induced by Nanoparticles – Standartisation of Methods within QualityNano FP7 Network

Co-authors NOVÁKOVÁ Zuzana, RÖSSNER Pavel, Jr., TOPINKA Jan
Collaboration: VSB – Technical University of Ostrava, Ostrava, Czech Republic, EU

Nanomaterials (NM) have become crucial for many applications promising significant improvement in various aspects of quality of life. Indisputable benefits of NM, however, might be discredited by concerns about potential adverse health effects arising from unique properties related to the nanosize. Safety assessment of NM is complicated by the fact that conventional methods are insufficient for evaluating their toxicity. Although new methods adapted for NM are being extensively introduced by toxicologists the lack of standardization causes inconsistency in existing nanotoxicological data making it impossible to draw general conclusions about NM toxicity. QualityNano is a current European FP7 project aimed at developing and implementing best practice and quality in nanosafety assessment. As one of the participating laboratories, we evaluated cytotoxic potential of two reference NM (non-cytotoxic 40nm carboxy-functionalized and cytotoxic 50nm amino-functionalized polystyrene nanoparticles) in lung adenocarcinoma A549 cell line using QualityNano Standard Operation Procedure for MTS Cell Viability Assay. Results of two performers showed good intra-laboratory concordance and obtained data were provided for inter-laboratory variation assessment which is currently being in progress within QualityNano Network. The same protocol was used to evaluate cytotoxic potential of the reference NM sample in immortalized bronchial epithelial BEAS-2B cell line. As expected, BEAS-2B cells were more susceptible than cancerous A549 cells. Finally, two other methods were employed to investigate concordance among various cytotoxicity assays. Both metabolic activity-based WST-1 assay and LDH membrane integrity assay have been proven to be relevant for studying NM cytotoxicity. ACKNOWLEDGEMENTS: Supported by the Czech Ministry of Education, Youth and Sports (COST.CZ, LD14002).