POSPÍŠILOVÁ Martina Contipro Biotech Ltd.

Polymer Encapsulated Upconverting Nanoparticles for Cell Imaging

Co-authors MRÁZEK Jiří, MATUŠKA Vít, HUERTA-ANGELES Gloria, DUSÍKOVÁ Monika, VELEBNÝ Vladimír

NaYF4-based upconverting nanoparticles (UCNPs) have recently emerged as promising nanomaterials for in vitro and in vivo bio-labelling and optical fluorescence imaging. UCNPs obtained by high-temperature decomposition methods are generally not dispersible in aqueous solutions and therefore, surface modification is crucial for further biological applications. Here we report a transfer of hydrophobic β-NaYF4:Er3+, Yb3+ UCNPs into water using amphiphilic hyaluronic acid. In aqueous media, oleyl-hyaluronic acid derivative could self-assemble into micelle-like structures into which oleate-capped β-NaYF4:Er3+, Yb3+ were incorporated via microemulsion method. Finally, a shell of these polymeric micelles was chemically cross-linked using bifunctional agent to ensure good separation of nanoparticles from the surrounding media. We demonstrated the uptake of encapsulated β-NaYF4:Er3+, Yb3+ by NHDF (Normal Human Dermal Fibroblasts) and MDA-MB 231 (human breast adenocarcinoma) cell lines, showing the utility of β-NaYF4:Er3+, Yb3+ UCNPs as bio-labels.

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