VASHISTH Priya Indian Institute of Technology Roorkee

Evaluation of Gellan based Electrospun Nanofibers for Wound Healing

Co-authors SINGH Harmeet, PRUTHI Parul A., SINGH Rajesh Pratap, PRUTHI Vikas

In recent years, specialized high technology materials such as polymeric nanofibers with biomimetic properties have emerged as a viable alternate to traditionally use simple natural coverings. In this investigation, wound healing ability of electrospun gellan-based nanofibers has been evaluated for the first time. The gellan-based ultrafine nanofibers were fabricated by using a blend mixture of gellan and polyvinyl alcohol (PVA). As determined by field emission scanning electron microscopy (FESEM), the fiber morphology and average diameter of the electrospun nanofiber was found to be influenced by solution viscosity, surface tension, specific gravity, flow rate, tip-to-collector distance and applied voltage. The equal ratio (1:1) of gellan-to-PVA was documented as an optimum polymeric ratio to fabricate uniform bead free nanofibers with an average diameter of 40±15.8nm. Differential scanning calorimetry (DSC) and FTIR analysis confirmed the stability as well as the crosslinking within the polymers. The crystalline nature of gellan-PVA nanofibers was assessed using X-ray diffraction (XRD) analysis. Furthermore, Human dermal fibroblast (HDF) cells were cultivated on fabricated blend nanofibers in order to investigate the effect of gellan-based nanofibers on the differentiation and proliferation of fibroblast cells. This study revealed that gellan-based nanofibers could be employed as a novel wound healing material.