MORAVEC Hynek Institute of Chemical Technology in Prague

Cell Interaction with Modified Titanium Nanostructures

Co-authors VANDROVCOVÁ Marta, CHOTOVÁ Kateřina, FOJT Jaroslav

Electrochemical oxidation of titanium allows preparing TiO2 tube layer with unique properties. This layer could be further modified to increase bioactivity. The aim of this work was to study the interaction of cells with various modified nanostructures on Ti6Al4V alloy. Nanotubes were prepared in a mixture of (NH4)2SO4 and NH4F.These surfaces were treated with calcium hydroxide solution, phosphoric acid and heat treated. Effect of each adjustment was controlled by SEM, XPS and Raman spectroscopy. Cell line SaOS-2 was used. Cells activities were determined on day 1, 3 and 7 after seeding. Cell spreading area was evaluated on day 1 after seeding. However, cells on covered samples displayed only slight reduce in comparison with polished titanium. The viability of the cells on all surfaces, evaluated by the LIVE/DEAD test, was higher than 90 % and there were no significant differences among the tested groups. The cells contained higher concentrations of vinculin, markers of adhesion, on both chemically treated nanostructures after 3 days of culturing. Further, marker of differentiation (osteocalcin) was measured on day 3 and 7 after seeding, respectively. Osteocalcin was homogeneously distributed on nanostructures with phosphorus. While heat treated surface did not promote activity of cells. Therefore, it can be concluded that chemically modified surfaces seem to be promising for biomaterials applications.