BAROŠOVÁ Hana VSB - Technical University of Ostrava

Treatment of Human Bodyfluid Samples for Phase Analysis

Co-authors TOMÁŠEK Vladimír, KUKUTSCHOVÁ Jana

Bodyfluid analysis is a novel approach for researching environmental pollution impact on human health. Bodyfluids are in touch with outer environment mostly through the respiratory tract, and thus inhalation of polluted air may cause the penetration of suspended solid particles through biomembranes. Solid particles are able to penetrate biomembranes due to their size below 100nm, therefore there is need for analytical methods, which are able to determine trace elements. Because of small amount of the obtained samples and potential infectivity of these samples, non-destructiveness of analytical method as well as the treatment of the samples is crucial. X-ray fluorescence spectroscopy (XRFS) is well known technique widely used for the determination of both major and trace elements in a wide range of materials. Raman microspectroscopy is also suitable method for phase analysis of bodyfluids. The aim of the study is focused on comparison of results from different human bodyfluids (mainly urine and amniotic fluid) treatment (various substrates) obtained by XRFS and Raman microspectroscopy. ACKNOWLEDGEMENTS: Authors thank to project reg. No. SP2014/76 and project Nanotechnology – the basis for international cooperation project, reg. no. CZ.1.07/2.3.00/20.0074 supported by Operational Programme 'Education for competitiveness' funded by Structural Funds of the European Union and state budget of the Czech Republic for financial support. Authors also thank to prof. MUDr. Daniela Pelclová, CSc. and doc. MUDr. Jana Dvořáčková, Ph.D. for providing the samples.