LIŠKOVÁ Jana Institute of Physiology AS CR v.v.i.

Effect of H- and O-termination of Nanocrystalline Diamond Films on Cell Adhesion and Osteogenic Differentiation

Co-authors BABCHENKO Oleg, VARGA Marian, KROMKA Alexander, HADRABA Daniel, SVINDRYCH Zdenek, BACAKOVA Lucie
Collaboration: Institute of Physics AS CR v.v.i., Prague, Czech Republic, EU

Nanocrystalline diamond (NCD) films are promising materials for tissue engineering, especially bone implants coating. Nanostructure and morphology of the NCD films can efficiently mimic properties of natural tissues. In addition, the NCD wettability can be tailored by grafting specific atoms (e.g., oxygen, hydrogen, etc.) which influences the final geometry and adhesion of proteins, and thus behaviour of cultivated cells. Therefore, the NCD films are proposed as multifunctional material for fundamental studies on the growth and adhesion of osteoblasts on bone implants, which is particularly our interest. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition. In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen (H-termination) or oxygen (O-termination) atoms. The human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion compared to H-terminated NCD films. Also the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Real-time PCR and immunostaining was higher on O-terminated films. The higher wettability of O-terminated NCD films (contact angle < 10°) is promising for adhesion and growth of osteoblasts. Besides, the O-terminated surface also seems to promote the osteogenic differentiation of the cultivated cells and production of extracellular matrix proteins. ACKNOWLEDGEMENTS: This work was supported within the Centre of Biomedical Research project (CZ.1.07/2.3.00/30.0025).