BERAN Milos Inovacentrum Czech Technical University in Prague

Immobilization of Microorganisms in Fibers

Co-authors DRAHORAD Josef, HUSEK Zdenek, TOMAN Frantisek

Probiotics have been defined as live microbes which transit the gastro-intestinal tract and in doing so benefit the health of the consumer. They have to be able to resist to gastric acidity and bile salts in the small intestine. A method for encapsulation and stabilization of probiotic yeast (Saccharomyces boulardii) or bacterial strains (Lactobacillus and Bifidobacterium spp.) in polymeric or biopolymeric fibers has been developed. Centrifugal methods using a rotating bell have been employed to produce a mixture of nano- and microfibers. Water solutions of polyvinylalcohol and gelatine with prebiotic fiber inulin and suspended microbial preparations have been used produce the fibers. The centrifugal spinning is simple, cheap and eco-friendly process. The process can be carried out at laboratory temperature without any heating. The survivability of probiotics in the fibrous preparations under in vitro conditions was conducted in a bile salts solution and a simulated gastric juice, followed by incubation. Acknowledgements The work was supported by the CZ1.05/3.1.00/14.0305 project of Ministry of Education, Youth and Sports of Czech Republic