SWAMI Rajan National Institute of Pharmaceutical Education and Research and CSIR-Indian Institute of Chemical Technology

Adenosine Receptors Ligand Modulated Nanoparticle targeting to Human Breast Cancer Cells via Over Expressed Adenosine Receptors

Co-authors SINGH Indu, KUMAR Manish, NAIDU V.G.M., KHAN Wahid, SISTLA Ramakrishna

G protein coupled cell surface adenosine receptors (ARs) are found to be up regulated in various tumor cells like breast, prostate and brain tumors. The present study was performed to investigate the efficiency of adenosine receptors ligand to target human breast cancer cell lines MCF-7. Solid lipid nanopartlces (SLN) were prepared by solvent emulsification and evaporation process and further evaluated by various techniques like X-Ray diffraction (XRD), Dynamic light scattering (DLS). Drug loaded SLN were surface modified with ARs ligand using carbodiimide coupling. Conjugation was confirmed using Infrared spectroscopy (IR). In vitro drug release was performed by dialysis bag method and conjugated SLN were found to give better sustain drug release as compared to unconjugated nanoparticles and drug solution. Cell toxicity assay were executed and results were encouraging with remarkable decrease in IC50 values as compared to drug encapsulated unconjugated lipidic nanoparticles and drug control and these results were further substantiated by improved cell uptake assay. Hence, this novel ARs ligand has the capability to target breast tumors and incorporating this ligand on surface of SLN modulates the delivery of nanoparticles specifically to ARs overexpressed tumos.

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