RAFIEIAN KOUPAEI Fatemeh Isfahan University of Technology

The extensive application of nanomaterials in industry, medicine and consumer products has raised concerns about their potential toxicity. The purpose of this study was to investigate the toxicologic responses of mice to cellulose nanofibrills (CNF). The animals were randomly divided into four experimental groups: Each group of five mice of each sex was administrated daily via oral gavage at dosage level of 0, 0.5, 1 and 2 g/kg b.w. CNF. Eighteen days later, the animals were anesthetized by ether and then sacrificed. In an effort to examine spleen, liver and kidney injury, we assessed coefficients of these organs, biochemical parameters of their functions, hematological indices, and histopathological changes in mice. CNF-treated mice showed no significant differences from controls neither with respect to the biochemical parameters nor in relation to blood elements of urea, creathinin (CR), alanine aminotransferase (ALT) and alkaline phosphatase (ALP). The uric acid (UA) level, a marker of renal function, and aspartate aminotransferase (AST) level, a hepatotoxicity indicator decreased and increased, respectively in mice dosed with CNF. These data suggested that hepatic damage occurred in treated groups. This was confirmed by histopathologic analyses which revealed that liver cell change (LLCC) and spotty necrosis of hepatocyte in the liver tissue occurred, although no sign of toxicity was observed in the spleen and kidney in histological examination. While there were no significant changes of the body weight coefficients of spleen and kidney, the liver coefficient increased gradually.